Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Supplier: Applied Biosystems™ 4333766F
![Encompass_Preferred](/content/dam/fishersci/glyphs/encompass_preferred.png)
Description
The Applied Biosystems™ Human B2M (beta-2-microglobulin) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan™ Primers and Probes.Assay Details:
Gene Symbol: B2M
RefSeq: NM_004048.2
Probe Exon Location:2-3
Amplicon Size: 75
Corresponding TaqMan Assay ID: Hs99999907_m1
TaqMan™ Endogenous Controls
Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.
Complete Solution for Quantitative Gene Expression
Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.
Simple to Use
All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.
Flexible Offering
We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
Choosing the Right Endogenous Control
Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.
Multiplex vs. Singleplex PCR
All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .
For Research Use Only. Not for use in diagnostic procedures.
![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
NM_004048.2 | |
Control Primer-Probe Set(s) | |
Tube | |
B2M | |
FAM | |
500 Reactions | |
Human | |
Gene Expression | |
500 Reactions |
75 | |
Primer-probe | |
2-step RT-qPCR | |
Sustainable packaging | |
FAM™, TaqMan™ | |
Room Temperature | |
B2M | |
20X |
Provide Content Correction
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?
Provide Content Correction