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Cytiva HiTrap™ Butyl-S FF Columns

Designed for purification of relatively strong hydrophilic molecules at low salt concentrations

Supplier:  Cytiva 17097813

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Catalog No. 45-000-174

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  • Useful for removal of strong hydrophobic contaminants, such as lipoproteins, lipids, pigments and more from biological samples
  • Used successfully for large-scale purification of recombinant Hepatitis B virus surface antigen (rec-HBsAg) from CHO cells
  • Robust production with high reproducibility



1 mL
7 mm
< 1 mL/min
5 x 1 mL
45 to 165 µm
6% Cross-linked Agarose
0.275 in.
HiTrap Butyl-S FF column
For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
5 bar [0.5 MPa] (70 psi)
3 to 13
4°C to 30°C, 20% Ethanol


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