Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

Supplier:  Gibco™ 12604013

Catalog No. 12-604-013

Description

Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

• Gentle on cells
• Room temperature-stable for 24 months
• Easy to use
• Animal origin-free
• Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts
• Can be directly substituted for trypsin in existing protocols
• Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

Energy efficient, less hazardous, sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Chelators: EDTA
• Cell Type: Mammalian Cells
• Concentration: 1 X
• Form: Liquid
• Sterility: Sterile-filtered
• Green Features: Energy efficient, Sustainable packaging
• pH: 6 to 8
• Quantity: 100 mL
• Shipping Condition: Room Temperature
• Tested For: In vitro Bioassay

• Content And Storage: Storage conditions: 15°C to 30°C, protect from light
  Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
  Shipping conditions: Room temperature
  Shelf life: 24 months from date of manufacture
• Classification: Animal Origin-free
• Culture Type: Adherent Cell Culture
• Product Type: Cell Culture Dissociation Reagent
• Without Additives: No Phenol Red
• Osmolality: Check and Record
• Product Line: Gibco™
• Remarks: Room Temperature Stable
• Source: Animal origin-free

Safety and Handling

ShelfLife : 24 Months

For Research Use Only. Not for use in diagnostic procedures.