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Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Supplier: Gibco™ 11875101

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Catalog No. 11-875-101

$15.13 / Each of 1

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Requires : Supplementation
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Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Cell Type	Leukemic Cells
Concentration	1 X
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility	Sterile-filtered
Without Additives	No HEPES, No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Quantity	100 mL

Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification	Animal Origin-free
Form	Liquid
Serum Level	Standard Serum Supplementation
With Additives	Glutamine, Phenol Red
Green Features	Sustainable packaging
Product Line	Gibco™
Shipping Condition	Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

WARNING: Cancer - www.P65Warnings.ca.gov

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