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Gibco™ RPMI 1640 Medium
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Supplier:  Gibco™ 11875085

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Catalog No. 11-875-085

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Requires : Supplementation
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Description

Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Leukemic Cells
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterile-filtered
No HEPES, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
1000 mL
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Liquid
Standard Serum Supplementation
Glutamine, Phenol Red
Sustainable packaging
Gibco™
Room Temperature
Recommendations

Recommendations

Safety and Handling

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications
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Promotions

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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.