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Gibco™ Recovery™ Cell Culture Freezing Medium

Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3

Supplier:  Gibco™ 12648010


Catalog No. 12-648-010

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For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

  • Suitable for a wide variety of mammalian cells
  • Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12
  • Quality tested for pH, osmolality, sterility, and endotoxin
  • Each lot is performance tested using CHO-K1 cells

Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR

Order Info

Shipped on dry ice.


Manufactured at a cGMP-compliant facility registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.



Storage conditions: -5 to -20°C. Protect from light.
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
Mammalian Cell Culture
Freezing Medium
No Phenol Red
Research Grade
Mammalian Cells
Standard Serum Supplementation
High Glucose, Phenol Red, DMSO (10%)
Gibco™, Recovery™
Dry Ice
Safety and Handling

Safety and Handling

ShelfLife : 12 Months



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For Research Use Only. Not for use in diagnostic procedures.