Gibco™ Medium 199, Earle's Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Catalog Number	Mfr. No.	Quantity
11-150-059	Gibco™ 11150059	500 mL
11-150-067	Gibco™ 11150067	10 x 500 mL
31-150-030	Gibco™ 31150030	10 x 500 mL

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Concentration: 1X
• Product Type: Medium 199
• Green Features: Sustainable packaging
• Product Line: Gibco™

• Form: Liquid
• Sterility: Sterile-filtered
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.