Gibco™ Ham's F-12 Nutrient Mix, powder

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Supplier:  Gibco™ 21700075

Catalog No. 21-700-075

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture
• Classification: Animal Origin-free
• Product Type: Ham's F-12 Nutrient Mixture
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 1 L

• Cell Type: Primary Rat Astrocytes
• Form: Powder
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Bicarbonate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 36 Months From Date of Manufacture

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.