Gibco™ DMEM, low glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
11-885-084	Gibco™ 11885084	500 mL
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11-885-092	Gibco™ 11885092	10 x 500 mL
11-885-076	Gibco™ 11885076	1000 mL
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Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: Low Glucose, L-Glutamine, Sodium Pyruvate, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

Order Info

Shipping Condition: Room Temperature

Specifications

• Classification: Animal Origin-free
• Form: Liquid
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

• Concentration: 1 X
• Sterility: Sterile-filtered
• Without Additives: No HEPES

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.