Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Gibco™ DMEM, high glucose, pyruvate

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.

$260.00 - $271.00

Specifications

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
View More Specs

Requires: Supplementation


Products 6
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
11-995-040
Encompass_Preferred
View Documents
Gibco™
11995040
1000 mL
Each for $50.75
N/A
Due to product restrictions, please to purchase or view availability for this product.
11-995-073
Encompass_Preferred
View Documents
Gibco™
11995073
10 x 500 mL
Case of 10 for $271.00
Add to Cart
 
11-995-065
Encompass_Preferred
View Documents
Gibco™
11995065
500 mL
Each for $31.50
N/A
Due to product restrictions, please to purchase or view availability for this product.
11-995-081
Encompass_Preferred
View Documents
Gibco™
11995081
6 x 1000 mL
Case of 6 for $260.00
Add to Cart
 
11-995-115
Encompass_Preferred
View Documents
Gibco™
11995115
5 L
Each for $317.00
N/A
Due to product restrictions, please to purchase or view availability for this product.
11-995-123
Encompass_Preferred
View Documents
Gibco™
11995123
10 L
Each for $496.00
N/A
Due to product restrictions, please to purchase or view availability for this product.
Description

Description

  • DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
  • Store at 2°C to 8°C, protected from light

Modified as follows:

  • With: High glucose, L-glutamine, phenol red, sodium pyruvate
  • Without: HEPES

Using DMEM

  • DMEM is unique as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Ambient
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
Sterile-filtered
No HEPES
Safety and Handling

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit