Gibco™ DMEM, high glucose, pyruvate

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.

Catalog Number	Mfr. No.	Quantity
11-995-040	Gibco™ 11995040	1000 mL
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11-995-073	Gibco™ 11995073	10 x 500 mL
11-995-065	Gibco™ 11995065	500 mL
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11-995-081	Gibco™ 11995081	6 x 1000 mL
11-995-115	Gibco™ 11995115	5 L
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11-995-123	Gibco™ 11995123	10 L
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Description

• DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
• Store at 2°C to 8°C, protected from light

Modified as follows:

• With: High glucose, L-glutamine, phenol red, sodium pyruvate
• Without: HEPES

Using DMEM

• DMEM is unique as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Classification: Animal Origin-free
• Form: Liquid
• With Additives: High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Shipping Condition: Ambient

• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Concentration: 1 X
• Sterility: Sterile-filtered
• Without Additives: No HEPES

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.