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Gibco™ DMEM, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.
$260.00 - $271.00
Specifications
Cell Line | HeLa, 293, Cos-7, and PC-12 |
---|---|
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
Classification | Animal Origin-free |
Concentration | 1 X |
Form | Liquid |
Requires: Supplementation
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
11-995-040
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Gibco™
11995040 |
1000 mL |
Each for $50.75
|
N/A | |||||
Due to product restrictions, please Sign In to purchase or view availability for this product. | |||||||||
11-995-073
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Gibco™
11995073 |
10 x 500 mL |
Case of 10 for $271.00
|
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11-995-065
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Gibco™
11995065 |
500 mL |
Each for $31.50
|
N/A |
Due to product restrictions, please Sign In to purchase or view availability for this product. | |||||||||
11-995-081
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Gibco™
11995081 |
6 x 1000 mL |
Case of 6 for $260.00
|
|
11-995-115
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Gibco™
11995115 |
5 L |
Each for $317.00
|
N/A |
Due to product restrictions, please Sign In to purchase or view availability for this product. | |||||||||
11-995-123
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Gibco™
11995123 |
10 L |
Each for $496.00
|
N/A | |||||
Due to product restrictions, please Sign In to purchase or view availability for this product. | |||||||||
Description
- DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
- Store at 2°C to 8°C, protected from light
Modified as follows:
- With: High glucose, L-glutamine, phenol red, sodium pyruvate
- Without: HEPES
Using DMEM
- DMEM is unique as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH
Order Info
Shipping Condition: Room Temperature
Specifications
HeLa, 293, Cos-7, and PC-12 | |
Animal Origin-free | |
Liquid | |
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate | |
Ambient |
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
1 X | |
Sterile-filtered | |
No HEPES |
Safety and Handling
ShelfLife : 12 Months From Date of Manufacture
When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.
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