Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
11-330-032	Gibco™ 11330032	500 mL
11-330-057	Gibco™ 11330057	10 x 500 mL
11-330-099	Gibco™ 11330099	5 L
11-330-107	Gibco™ 11330107	10 L

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:
With: L-glutamine, HEPES, Phenol Red

The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• With Additives: High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.