GFP Monoclonal Antibody (GF28R), HRP, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA515256HRP

Catalog No. MA515256HRP

Description

MA5-15256-HRP has been successfully used in Western blotting applications with GFP tagged samples.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: antibody with 0.07% Kathon
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: GF28R
• Conjugate: HRP
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.
• Quantity: 50 μL
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Form: Liquid
• Target Molecule: Tag