Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 56996642

Catalog No. 56-996-642

Description

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation. Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein. Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00). Crossreactivity in rhesus monkeys has been published. Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This TWAJ antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product No.

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

Specifications

• Antigen: Gata-3
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
• Gene Accession No.: P23771, P23772
• Gene Symbols: GATA3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14462, 2625, 713840, 733631
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: TWAJ
• Conjugate: Alexa Fluor 700
• Gene: GATA3
• Gene Alias: EGK_19445, GATA binding factor-3, GATA binding protein 3, GATA binding protein-3, GATA3, Gata-3, GATA-binding factor 3, GATA-binding protein 3, HDR, HDRS, jal, MGC2346, MGC5199, MGC5445, Trans-acting T-cell-specific transcription factor GATA-3, Transacting T-cell-specific transcription factor GATA-3
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Porcine, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG2b κ

Safety and Handling

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