GAPDH Recombinant Rabbit Monoclonal Antibody (SA30-1), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific MA531976

Catalog No. PIMA531976

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

Specifications

• Antigen: GAPDH
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
• Gene Accession No.: O14556, P04406, P04797, P16858, Q5MJ86, Q5XJ10, Q64467, Q9ESV6
• Gene Symbols: GAPDH, GAPDHS
• Immunogen: Recombinant protein within mouse GAPDH aa 94-333
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Zebrafish, Rat, Zebrafish
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Western Blot
• Clone: SA30-1
• Conjugate: Unconjugated
• Gene: GAPDHS
• Gene Alias: 38 kDa BFA-dependent ADP-ribosylation substrate, aging-associated gene 9 protein, BARS-38, bb02e05, cb350, cb609, CDABP0047, EC 1.2.1.12, epididymis secretory protein Li 278, epididymis secretory sperm binding protein Li 162eP, fb71f08, fk58c09, G3PD, G3PDH, GAPD, GAPD2, gapdh, GAPDH2, GAPDH-2, GAPDHS, Gapds, Gapd-s, glceraldehyde-3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase, testis-specific, glyceraldehyde phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), glyceraldehyde-3-phosphate dehydrogenase 2, glyceraldehyde-3-phosphate dehydrogenase GAPDH, glyceraldehyde-3-phosphate dehydrogenase like-17 protein, glyceraldehyde-3-phosphate dehydrogenase type 2, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic, glyceraldehyde-3-phosphate dehydrogenase, testis-specific, glyceraldehyde-phosphate-dehydrogenase, glycerine aldehyde 3-phosphate dehydrogenase, HEL-S-
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 14433, 14447, 24383, 2597, 26330, 317743, 406367, 66020
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid