Galectin 9 Monoclonal Antibody (RG9-35), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  eBioscience 25921182

Catalog No. 25-921-182

Description

Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family. Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells. Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.

Specifications

• Antigen: Galectin 9
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O08573
• Gene Symbols: Lgals9
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16859
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: RG9-35
• Conjugate: PE-Cyanine7
• Gene: Lgals9
• Gene Alias: 36 kDa beta-galactoside-binding lectin; AA407335; AI194909; AI265545; ecalectin; Gal9; gal-9; galectin 9; Galectin9; galectin-9; HOM HD 21; HUAT; Lectin galactose binding soluble 9 (Galectin-9); lectin, galactose binding, soluble 5; lectin, galactose binding, soluble 9; Lectin, galactose binding, soluble 9 (Galectin-9); lectin, galactoside binding soluble 9; lectin, galactoside-binding, soluble, 9; LGALS35; Lgals5; LGALS9; LGALS9A; tumor antigen HOM-HD-21; UAT; urate transporter/channel; urate transporter/channel protein
• Host Species: Rat
• Quantity: 100μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ