Anti-Human Foxp3 Staining Set APC, eBioscience™

Antibody Staining Kit

Supplier:  Invitrogen 77577440

Catalog No. 50-169-79

Includes: Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.
Fixation/Permeabilization Diluent (cat. 00-5223): 100mL, store at 2-8°C.
Permeabilization Buffer (10X) (cat. 00-8333): 100mL, store at 2-8°C. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
Normal Mouse Serum (cat. 24-5544): 100 μL, store at 2-8°C.
Anti-Human Foxp3 APC (clone 236A/E7, cat. 17-4777): 25 tests, store at 2-8°C. Light-sensitive material.

Description

This Anti-Human Foxp3 Staining Set contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells. The 236A/E7 monoclonal antibody reacts with human Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

The 236A/E7 antibody has been shown to crossreact with rhesus macaque, sooty mangabey, and cynomolgus Foxp3. 236A/E7 recognizes a different epitope of Foxp3 than PCH101 and 150D/E4.

Specifications

• Antigen: Foxp3
• Classification: Monoclonal
• Conjugate: APC
• Host Species: Mouse
• Regulatory Status: RUO
• Target Species: Cynomolgus, Human, Macaque, Monkey, Non-human Primate, Rhesus Monkey
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: 236A/E7
• Gene Alias: Forkhead Box P3, Scurfin, JM2
• Quantity: Kit
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C. Light-sensitive material. Use within 6 months of opening or by date indicated on the bottle.
• Product Line: eBioscience™

For Research Use Only. Not for use in diagnostic procedures.