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Fisher BioReagents™ Recombinant DNase 10X RXN Buffer, Optizym™, Fisher BioReagents
Ideal for use in RT-PCR and other RNA applications
This item is not returnable.
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Description
- Efficiently hydrolyzes phosphodiester linkages of double- or single-stranded DNA
- Requires magnesium and calcium for maximal activity
- Tested for RNase activity, protease activity, and in specific performance tests
- Storage Buffer Components: 20mM HEPES (pH 7.5), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% (v/v) glycerol
- Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5), 25mM MgCl2, 5mM CaCl2
- Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg DNA in 10 minutes at 37°C
- Unit present in each tube: 1mL per ampule
Specifications
| 2 U/μL | |
| 20mM HEPES (7.5 pH), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% Glycerol | |
| 1000 U | |
| Liquid |
| RT-PCR, other RNA applications | |
| -20°C | |
| OPTIZYME™ rDNase I |
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