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Abnova™ FGFR1/CEN8 CISH Probe
FGFR1/CEN8 CISH Probe is designed for the qualitative detection of the human FGFR1 gene and chromosome 8 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Supplier: Abnova™ CG0007
Description
The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (FGFR1 gene region), and dinitrophenyl-labeled polynucleotides appear as bright red colored region distinct dots (CEN 8).
Normal situation: In interphases of normal cells or cells without an amplification involving the FGFR1 gene locus, two green signals and two red signals appear
Aberrant situation: In a cell with amplification of the FGFR1 gene locus, multiple copies of the green signal or green signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.
Specifications
| The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use. | |
| CISH-P |
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