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Thermo Scientific™ T7 RNA Polymerase, HC (200 U/μL)
Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Supplier: Thermo Scientific™ EP0113
Description
Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter.
It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Recommended for:
Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.
Note:
Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.
Specifications
| T7 RNA Polymerase | |
| 25,000 U | |
| >200U/µL |
Safety and Handling
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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