Thermo Scientific™ SP6 RNA Polymerase (20 U/μL)

Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Supplier:  Thermo Scientific™ EP0131

Catalog No. FEREP0131

Description

Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.

• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Specifications

• Polymerase: SP6 RNA Polymerase
• Quantity: 2000 U
• Concentration: 1X

For Research Use Only. Not for use in diagnostic procedures.