Thermo Scientific™ Genomic DNA Purification Kit

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

Supplier:  Thermo Scientific™ K0512

Catalog No. FERK0512

Description

Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Specifications

• Content And Storage: • 40 mL Lysis Solution; room temperature
  • 8 mL Precipitation Solution (concentrated); room temperature
  • 10 mL NaCl Solution; room temperature
  
  
• Sample Type: Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
• Final Product Type: gDNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Starting Material Amount: Bacteria: ≤20 mg
  Cells: ≤10⁶
  Plant: ≤100 mg
  Tissue: ≤30 mg
  Serum: ≤200 μL
  Whole Blood: ≤200 μL

• Isolation Technology: Organic Extraction
• Elution Volume: 20 to 100 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 100 Preps
• Test Time: 25 min.

For Research Use Only. Not for use in diagnostic procedures.