Learn More
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
$126.60 - $317.65
Specifications
Concentration | 1 U/μL |
---|---|
Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Product Type | Alkaline Phosphatase |
Description
FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.
FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C . Therefore, removal of alkaline phosphatase is not required prior to ligation.
Highlights
- Recombinant enzyme
- Fast dephosphorylation -10 minutes at 37°C
- Fast and complete inactivation -5 minutes at 75°C
- Simultaneous digestion and dephosphorylation of vector DNA
- 100% active in restriction enzyme and PCR buffers
- One protocol for all types of DNA ends:
- 5'-overhangs
- 3'-overhangs
- blunt-ends
- single nucleotides
- PCR clean-up in conjunction with Exo I
- Protein dephosphorylation
Specifications
1 U/μL | |
Phosphatase | |
PCR Buffer, Enzyme Buffer | |
Alkaline Phosphatase |
For Research Use Only. Not for use in diagnostic procedures.
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.