Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Catalog Number	Mfr. No.	Quantity
PIA39257	Thermo Scientific™ A39257	10 x 1 mg
PI21335	Thermo Scientific™ 21335	100 mg

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Specifications

• Product Type: Sulfo-NHS-LC-Biotin
• Cell Permeability: Cell-Impermeant
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Spacer: Mid-length

• Product Line: EZ-Link™
• Chemical Reactivity: Amine
• Label or Dye: Biotin

For Research Use Only. Not for use in diagnostic procedures.