Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Supplier:  Thermo Scientific™ 21425

Catalog No. PI21425

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

• Shorter spacer arm than Sulfo-NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
• Negative charge of -SO₃ keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
• Water solubility eliminates the need for harmful organic solvents

U.S. Patents 5,892,628 and 5,872,261.

Specifications

• Description: EZ-Link Sulfo-NHS-Biotinylation Kit
• Content And Storage: Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
  • EZ-Link Sulfo-NHS-Biotin, 25 mg
  • PBS Pack (makes 500mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
  • HABA Solution, 1 mL
  • Avidin, 10 mg
  
  Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C.
• Product Line: EZ-Link™
• Label or Dye: Biotin

• Product Type: Sulfo-NHS-Biotinylation Kit
• Labeling Scale: 1 to 10 mg
• Solubility: Water
• Labeling Target: Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.