Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Catalog Number	Mfr. No.	Quantity
PIA39256	Thermo Scientific™ A39256	10 x 1 mg
PI21217	Thermo Scientific™ 21217	50 mg

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
• Very short - spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.

Specifications

• Product Type: Sulfo-NHS-Biotin
• Cell Permeability: Cell-Impermeant
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Spacer: Short

• Product Line: EZ-Link™
• Chemical Reactivity: Amine
• Label or Dye: Biotin

For Research Use Only. Not for use in diagnostic procedures.