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Gibco™ ExpiFectamine™ CHO Transfection Kit

These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells cultured in ExpiCHO™ Expression Medium

Supplier:  Gibco™ A29131


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Catalog No. A29131

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  • Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
  • Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
  • Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
  • Delivers robust and reproducible transfection results, giving you greater confidence in your results
  • Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L


CHO Transfection Kit
Five 10-L kits; sufficient for transfection of 50 L of culture

160 mL ExpiFectamine CHO Reagent
300 mL ExpiFectamine CHO Enhancer
16.5 L ExpiCHO Feed

Store in the dark at 2 -8°C.
Ovarian Cells, Established Cell Lines
Lipid-Based Transfection
Animal Origin-Free, Chemically Defined
Kit Includes: 160mL ExpiFectamine™ CHO Reagent, 300mL ExpiFectamine™ CHO Enhancer, 16.5L ExpiCHO Feed
For 50L of culture
Room Temperature


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