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Gibco™ ExpiFectamine™ CHO Transfection Kit

These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells cultured in ExpiCHO™ Expression Medium

Supplier:  Gibco™ A29130


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Catalog No. A29130

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  • Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
  • Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
  • Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
  • Delivers robust and reproducible transfection results, giving you greater confidence in your results
  • Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L


Ovarian Cells, Established Cell Lines
For 10L of culture
Lipid-Based Transfection
32 mL ExpiFectamine CHO Reagent
60 mL ExpiFectamine CHO Enhancer
3.3 L ExpiCHO Feed

Sufficient for transfection of 10 L of culture

Store in the dark at 2 -8°C.
Animal Origin-Free, Chemically Defined
CHO Transfection Kit
Room Temperature
Kit Includes: 32 mL ExpiFectamine™ CHO Reagent, 60 mL ExpiFectamine™ CHO Enhancer, 3.3 L ExpiCHO Feed


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