Gibco™ ExpiFectamine™ CHO Transfection Kit

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.

Supplier:  Gibco™ A29130

Catalog No. A29130

Description

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001).

All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.

Features of the ExpiFectamine CHO Transfection Kit include:

• Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discovery
• Achieve >90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Uses one-third to one-half less plasmid DNA than other transfection reagents
• Scale growth and transfection in culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately.

Specifications

• Cell Line: ExpiCHO-S™, CHO-S, SFM Adapted
• Content And Storage: Kit includes:
  • ExpiFectamine CHO Reagent, 32 mL
  • ExpiFectamine CHO Enhancer, 60 mL
  • ExpiCHO Feed, 3.3 L
  
  Sufficient for transfection of 10 L of culture
  Store in the dark at 2°C to 8°C.
• Transfection Technique: Lipid-based Transfection
• Product Type: CHO Transfection Kit
• Shipping Condition: Room Temperature

• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Cell Type: Ovarian Cells
• Product Line: ExpiFectamine™
• Quantity: For 10 L of culture

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.