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Gibco™ Expi293™ Expression Medium
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Expi293™ Expression Medium
Supplier: Gibco™ A1435101
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Description
Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.- Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
- Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
- Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
- Supports growth and transfection of 293 cells in culture formats of less than 1mL in multiwell plates to greater than 10L in disposable bioreactors
- Does not contain phenol red
Specifications
Store in the dark at +2°C to +8°C | |
293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F | |
Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
Suspension Cell Culture | |
Expression Medium | |
Sterile | |
No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol | |
Bottle(s) | |
No antibiotics | |
275-295 mOsm/kg | |
Cell Culture Grade |
CO2 | |
Embryonic Kidney Cells | |
1 X | |
Liquid | |
Serum-free | |
High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl | |
< 1 EU/ml | |
Sustainable packaging | |
Calcium, Magnesium, Sodium Phosphate | |
Expi293™ | |
Room Temperature |
Safety and Handling
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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