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Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Supplier: Thermo Scientific™ EN0582
Description
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
- Active in PCR buffers
Applications
- Primer removal from PCR mixtures:
- prior to PCR product sequencing li>for one-tube "megaprimer" PCR mutagenesis
Note
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
Specifications
20 U/μL | |
Exonuclease | |
20,000 U | |
Exonuclease I |
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For Research Use Only. Not for use in diagnostic procedures.