MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys4)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Supplier:  MilliporeSigma™ 17614

Catalog No. 17-614-MI

Description

Specifically detects Trimethyl-Histone H3 (Lys4)α Clone: in Human, Mammalia, Mouse samples, and it is validated for ChIP, ChIP-seq, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys4)α
• Classification: Monoclonal
• Formulation: Anti-Trimethyl-Histone H3 (Lys4) recombin-ant rabbit monoclonal IgG. One vial containing 75μL of protein A purified rabbit IgG in storage buffer (0.1M Tris-Glycine pH 7.4, 0.15M NaCl, 0.05% NaN3, with the addition of 40% glycerol.
  Normal Rabbit IgG. One vial containing 75μL of normal rabbit IgG.
  Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Symbols: H3F3A, H3F3B, H3F3
• Immunogen: The trimethyl-histone H3 (Lys4) antibody is made against a BSA-conjugated, synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Trimethyl-Histone H3 (Lys4)
• Target Species: Human, Mammalia, Mouse
• Form: Purified

• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q66I33
• Host Species: Rabbit
• Quantity: 25 Assays
• Research Discipline: Epigenetics, Nuclear Function
• Gene ID (Entrez): NM_003493
• Includes: Trimethyl-Histone H3 (Lys4) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K4Me3.
• Content And Storage: −20°C for one year from date of shipment
• Isotype: IgG

For Research Use Only