MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 17622

Catalog No. 17-622-MI

Description

Specifically detects Trimethyl-Histone H3 (Lys27) Clone: in Human, Mouse samples, and it is validated for ChIP, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys27)
• Classification: Polyclonal
• Formulation: Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg purified Rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers human alpha-Satellite. One vial containing 75μL of 5μM of each control primer specific for human alpha-Satellite. FOR: CTG CAC TAC CTG AAG AGG AC; REV: GAT GGT TCA ACA CTC TTA CA
• Gene Symbols: H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B
• Immunogen: KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: trimethyl-Histone H3 (Lys27)
• Target Species: Human, Mouse
• Form: Purified

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q16695
• Host Species: Rabbit
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_003484
• Includes: Trimethyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.
• Content And Storage: −20°C for one year

For Research Use Only