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MilliporeSigma™ Upstate™ Sox-2, clone 6F1.2α, 6F1.2, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 17656

Catalog No. 17-656-MI


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Description

Description

Specifically detects Sox-2, clone 6F1.2α Clone: 6F1.2 in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, Western Blotting

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable 'per ChIP' reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
Specifications

Specifications

Sox-2, clone 6F1.2α
Monoclonal
Unconjugated
P48431
Mouse
25 Assays
Epigenetics and Nuclear Function
NP_003097
This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
IgG2b κ
ChIP assay, Dot Blot, Immunocytochemistry, Western Blot
6F1.2
Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG). One vial containing 100μg of protein A purified antibody in 140μL of 0.02M phosphate buffer pH 7.6 with 0.25M NaCl containing 0.1% sodium azide, and 30% glycerol. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
SOX2; ANOP3; MGC2413; MCOPS3
Recombinant GST Fusion protein.
RUO
Primary
Recognizes Sox-2, MW: ∽34kDa.
Human, Mouse
Purified
SDS
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