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MilliporeSigma™ Upstate™ RNA Pol IIα, Mouse monoclonal, ChIP Validated Antibody and Primer Set
GSA_VA

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 17620

Catalog No. 17-620-MI


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Description

Description

Specifically detects RNA Pol IIα Clone: in Human, Mouse, Rat, S. cerevisiae, Yeast samples, and it is validated for ChIP, Immunofluorescence, Immunoprecipitation

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome

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Specifications

Specifications

RNA Pol IIα
Monoclonal
Anti-RNA Pol II (mouse monoclonal IgG1,purified). One vial containing 25μg of purified antibody in 25μL volume. Normal Mouse IgG. One vial containing 25μg of mouse IgG in 25μL volume. Control Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Mouse
Protein G purified
RUO
Primary
Recognizes RNA Pol II
Human, Mouse, Rat, S. cerevisiae, Yeast
Purified
ChIP assay, Immunofluorescence, Immunoprecipitation
Unconjugated
P24928
The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II.
25 Assays
Epigenetics and Nuclear Function
NP_000928
RNA Pol II ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of RNA Pol II.
−20°C for one year from date of shipment
IgG1
SDS
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