MilliporeSigma™ Upstate™ N-CoR, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Rabbit Polyclonal Antibody
Supplier: MilliporeSigma™ 1710260
Specifically detects N-CoR Clone: in Human, Mouse samples, and it is validated for ChIP, Western BlottingIn the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.
|Anti-N-CoR (Rabbit Polyclonal). One vial containing 75μg of affinity purified polyclonal in buffer containing 0.1M Tris-glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL. Normal Rabbit IgG One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, p21. One vial containing 75μL of each primer (5μM) specific for the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: CCC ACA GCA GAG GAG AAA GAA; REV: CTG GAA ATC TCT GCC CAG ACA|
|KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.|
|Epigenetics and Nuclear Function|
|ChIP assay, Western Blot|
|This ChIPAb+ N-CoR -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.|
|-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles|
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