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MilliporeSigma™ Upstate™ Histone H3 (Unmodified Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Mouse Monoclonal Antibody
Supplier: MilliporeSigma™ 17675
Description
Specifically detects Histone H3 (Unmodified Lys4)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting
Specifications
Histone H3 (Unmodified Lys4)α | |
Monoclonal | |
Anti-Histone H3 (Unmodified Lys4) (mouse monoclonal IgG2bκ, clone CMA301). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers β-Globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC | |
HIST3H3; H3FT; H3/t; H3t; H3/g; H3.4; H3T | |
The histone H3 (Unmodified Lys4) purified antibody is made against a synthetic peptide corresponding to amino acids 1-12 of histone H3. | |
25 Assays | |
Epigenetics and Nuclear Function | |
NM_003493 | |
This ChIPAb+ Histone H3 (Unmodified Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. | |
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles | |
IgG2b κ |
ChIP Assay, Western Blot | |
Unconjugated | |
Q66I33 | |
Mouse | |
Protein G purified | |
RUO | |
Primary | |
Recognizes unmodified histone H3 (Lys4), Mr 17kDa. Epitope specificity of antibody as determined by ELISA maps near Lys4. Antibody tolerates modifications on Lys9 and Ser10 but not Lys4 or Thr31. | |
Vertebrates | |
Purified |
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