MilliporeSigma™ Upstate™ CTCFα, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Mouse Monoclonal Antibody
Supplier: MilliporeSigma™ 1710044
Specifically detects CTCFα Clone: in Human samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Multiplexing, Western BlottingCTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.
|Anti-CTCF, (mouse monoclonal IgG1κ. One vial containing 50μg of protein G purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide, before the addition of glycerol to 30%. Normal Mouse IgG. Two vials containing 25μg purified IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, Human H19 ICR. One vial containing 75μL of 5μM of each primer specific for human H19 internal control region. FOR: CCC ATC TTG CT GACC TCAC; REV: AGA CCT GGG ACG TTT CTG TG|
|His-tagged recombinant protein corresponding to human CTCF.|
|Epigenetics and Nuclear Function|
|The ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG) and qPCR primers which amplify a 165bp region of human H19 internal control region.|
|-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles|
|Multiplex, Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunoassay, Western Blot|
|Protein G purified|
|Recognizes CTCF, Mr ∽130kDa.|
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