MilliporeSigma™ Upstate™ CREBα, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Supplier:  MilliporeSigma™ 17600

Catalog No. 17-600-MI

Description

Specifically detects CREBα Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Western Blotting

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene. The CREB and negative control cultured supernatant are supplied in a scalable per ChIP reaction size and can be used to functionally validate the precipitation of CREB associated chromatin.

Specifications

• Antigen: CREBα
• Classification: Monoclonal
• Formulation: 25 assays per set. Recommended use: ∽10μL antibody per chromatin immunoprecipitation (dependent upon biological context).
• Gene Symbols: CREB1, MGC9284, CREB
• Immunogen: CREB purified antibody is made against a KLH-conjugated synthetic peptide corre-sponding to amino acids 5-24 of human CREB.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes CREB, MW ∽43kDa.
• Target Species: Human, Mouse, Rat
• Form: Culture Supernatant

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P16220
• Host Species: Rabbit
• Quantity: 25 Assays
• Research Discipline: Epigenetics, Nuclear Function
• Gene ID (Entrez): NP_004370
• Includes: This ChIPAb+ CREB -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG

For Research Use Only