Abnova™ EGFR CISH Probe

EGFR CISH Probe is designed for the qualitative detection of human EGFR gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0017

Catalog No. 89-335-564

Description

The probe is intended to be used in combination with the CISH Implementation Kit 1, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots.
Normal situation: In interphases of normal cells or cells without an amplification involving the 7p11.2 band, two distinct dot-shaped brown signals per nuclei appear.
Aberrant situation: In a cell with amplification of the EGFR gene locus or aneuploidy of chromosome 7, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-P,CISH-Ce
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• Includes: Reagent Provided:
  
  1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 7p11.2-7p12* (chr7:55,034,991-55,380,617) harboring the EGFR gene
  2. Formamide based hybridization buffer
  
  *according to Human Genome Assembly GRCh37/hg19