Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Edvotek™ Western Blot Analysis, Polyacrylamide-Based
In Western Blot analysis, protein identification is based on antibody and antigen reactions.
Supplier: Edvotek™ 317
Includes:
- Instructions
- Negative control
- All samples and antibodies
- Various reagents and buffers
- Membrane and filter paper
Requires :
- Three polyacrylamide gels (12%)
- Vertical gel electrophoresis apparatus
- Power supply
- Automatic micropipet with fine tips
- Laboratory glassware
- Metric rulers
- Distilled or deionized water
- Glacial acetic acid
- Methanol
Description
- Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane
- Membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme
- Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band
- Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight
Experiment times:
- Electrophoresis, one hour
- Blot, overnight
- Detection, two hours and 30 minutes
Kit does not require an electrotransfer apparatus.
Order Info
Contains materials for six blots.
Specifications
| Western Blot Kit | |
| Instructions, negative control, all samples and antibodies, various reagents and buffers, membrane and filter paper | |
| 2.5 hr. |
| Proteins are separated on polyacrylamide gels and are transferred (blotted) to a nylon membrane | |
| 6 blots | |
| Refrigerated |
Provide Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Provide Content Correction