Edvotek™ Purification of the Restriction Enzyme Eco RI

In this Edvotek experiment, students actually purify the restriction enzyme, Eco RI.

Supplier:  Edvotek™ 302

Catalog No. S05656

Includes:

• Instructions
• Ion exchange matrix
• Chromatography columns
• E. coli cell extract
• Equilibration and elution buffer
• Lambda DNA
• Lambda/Eco RI Marker
• KCl
• Glycerol
• Dilution and reaction buffers
• Gel loading solution
• Agarose
• Electrophoresis buffer
• Stain

Description

In this experiment, students actually purify the restriction enzyme, Eco RI. This procedure utilizes an ion exchange chromatography step for Eco RI purification.

 

• Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis
• Fractions that contain Eco RI are identified and pooled
• Total and specific activities are calculated
• Recommended for college level courses

 

Experiment times:

• Packing column, 45 minutes
• Restriction analysis A, 35 minutes
• Restriction analysis B, 50 minutes
• Gel Prep, 30 minutes
• Electrophoresis, 30 minutes
• Staining and destaining, two minutes

 

All You Need

 

• horizontal gel electrophoresis apparatus
• power supply
• UV visualization system
• waterbath
• microcentrifuge
• microwave or hot plate
• UV spectrophotometer & cuvettes
• automatic micropipet with tips
• ring stands & clamps
• 10 ml pipets
• lab glassware
• ice and ice buckets.

 

Order Info

For five purifications

Recommended Storage

Some components requre freezer storage

Specifications

• Product Type: Purification Kit
• Includes: Instructions, ion exchange matrix, chromatography columns, E. coli cell extract, equilibration and elution Buffer, Lambda DNA, Lambda or Eco RI Marker, KCl, glycerol, dilution and reaction Buffers, gel loading solution, Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide
• Time Range: 3 hours 15 minutes

• For Use With (Application): Purify the restriction enzyme, eco ri
• Quantity: 5 purifications
• Content And Storage: Refrigerated. Some components require freezer storage.