Edvotek™ Purification and Size Determination of Green and Blue Fluorescent Proteins

The unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E. coli extract.

Supplier:  Edvotek™ 255

Catalog No. S05651

Includes:

• Instructions
• Columns and matrix
• GFP and BFP extracts
• Buffer
• Protein gel reagents for optional activity

Description

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins.

 

• Unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract
• Column fractions containing GFP or BFP are identified by fluorescence and then purified
• As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis to estimate the purity and size of the GFP and BFP proteins

 

Experiment times:

• Packing and running column, 45 minutes
• Optional electrophoresis, one hour
• Staining, 30 minutes
• Destaining, two hours

 

All You Need

 

• water bath
• long wave UV lamp
• ring stand; clamps
• automatic micropipet
• vertical gel electrophoresis apparatus
• power supply
• hot plate
• polyacrylamide gels (12%)

 

Order Info

For six lab groups.

Recommended Storage

Some components require freezer storage

Specifications

• Product Type: Green and Blue Fluorescent Protein Kit
• Includes: Instructions, columns and matrix, gfp and bfp extracts, buffer, protein gel reagents for optional activity
• Content And Storage: Refrigerated. Some components require freezer storage.

• For Use With (Application): Used as an assay during their purification from an e. coli extract
• Time Range: 4 hours 15 minutes; Staining time: 30 minutes
• Class Size: 6 Lab Groups