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Edvotek™ Edvotek™ Lighting Up Life: Expression of GFP in C. elegans Experiment Kit
Students use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).
Supplier: Edvotek™ 858
Includes: Instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM Medium Salts, UltraSpec-Agarose™ powder, electrophoresis buffer (50x), 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops
Requires : Covered box, fluorescent and dissecting microscopes, timers, waterbath, incubator (optional), thermal cycler or three waterbaths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or white light visualization system, automatic micropipets with tips, microwave or hot plate, pipet pump, flasks or beakers, hot gloves, distilled or deionized water, ice buckets and ice
Description
Scientists can directly manipulate an organism's genome to produce a phenotype using engineered genes called transgenesDetailed time:
- Growing Bacteria : Overnight
- Plating Worms: 15 minutes
- Worm Growth: 3-4 Days
- Set Up: 30 minutes
- PCR: 2 hours or overnight
- Electrophoresis: 90 minutes
Specifications
| Instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM Medium Salts, UltraSpec-Agarose™ powder, electrophoresis buffer (50x), 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops | |
| Lighting Up Life: Expression of GFP in C. elegans experiment kit | |
| Some Components Require Refrigerator & Freezer Storage | |
| 10 Lab Groups |
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