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Edvotek™ Edvotek™ Lighting Up Life: Expression of GFP in C. elegans Experiment Kit
SCI_ED

Students use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).

Supplier:  Edvotek™ 858

Catalog No. S20858


Includes: Instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM Medium Salts, UltraSpec-Agarose™ powder, electrophoresis buffer (50x), 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops 

Requires : Covered box, fluorescent and dissecting microscopes, timers, waterbath, incubator (optional), thermal cycler or three waterbaths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or white light visualization system, automatic micropipets with tips, microwave or hot plate, pipet pump, flasks or beakers, hot gloves, distilled or deionized water, ice buckets and ice

Description

Description

Scientists can directly manipulate an organism's genome to produce a phenotype using engineered genes called transgenes


Detailed time:
  • Growing Bacteria : Overnight
  • Plating Worms: 15 minutes
  • Worm Growth: 3-4 Days
  • Set Up: 30 minutes
  • PCR: 2 hours or overnight
  • Electrophoresis: 90 minutes
Specifications

Specifications

Lighting Up Life: Expression of GFP in C. elegans Experiment Kit
Instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM Medium Salts, UltraSpec-Agarose™ powder, electrophoresis buffer (50x), 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops
Some Components Require Refrigerator & Freezer Storage
10 Lab Groups
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