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Edvotek™ DNA Fingerprinting Using Restriction Enzymes
Learn about restriction enzymes.
Supplier: Edvotek™ 225
Includes: instructions, crime scene and suspect Ready-to-Load™ DNA samples, DNA Standard Marker, Dryzymes™ - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, Enzyme Grade Water, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, InstaStain™ Blue Cards, FlashBlue™ DNA Stain, Disposable Pipets, & Microcentrifuge Tubes
Requires :
Required: Horizontal gel electrophoresis apparatus; DC power supply; Automatic micropipets with tips; Water bathHotplate, Bunsen burner or microwave oven
Recommended: DNA visualization system (white light); Staining tray and net; 250mL flasks; Pipet pump; Hot gloves; Marking pens;Distilled or deionized water; Ice
Description
- The objective of this simulated forensic analysis is to develop an understanding of DNA fingerprinting by use of restriction enzymes
- Hypothetical crime scene samples are provided in addition to DNA samples, restriction enzymes and reaction buffer to digest suspect's DNA
- Digestion products are separated by agarose gel electrophoresis
- Students determine the guilty suspect through identification of the unique restriction enzyme fragmentation patterns
- Does not contain human DNA
- Some components require refrigeration, some require freezing
Order Info
Contains materials for six gels (six lanes each) with InstaStain™ Methylene Blue.
Specifications
DNA Fingerprinting Using Restriction Enzymes Classroom Kit | |
6 Gels | |
1.45 hr. |
For dna fingerprinting | |
Electrophoresis apparatus; power supply; automatic pipet with tips; waterbath; balance; microwave or hot plate; visualization (white light); misc. labware; pipet pumps or bulbs; metric rulers; floating racks; photodocumentation system (optional) | |
Some components require refrigeration and some require freezing |
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