Edvotek™ Diversity of Fish Proteins

Determine the relationship between three fish species based on their protein profiles.

Supplier:  Edvotek™ 253

Catalog No. S05650

Includes: Instructions, fish LyphoProtein™ samples, protein molecular weight standards, practice gel-loading solution, buffer and Protein InstaStain™

Description

Study the diversity of fish with these prestained, lyophilized proteins.

 

• Students extract and prestain total protein from perch, walleye and salmon using an indicator dye
• After separating each fish protein sample by denaturing SDS-polyacrylamide gel electrophoresis, students find a characteristic banding pattern Pattern can be used to identify the specific species

 

Experiment times:

• Electrophoresis, one hour
• Staining, 20 minutes
• Destaining, two hours

 

All You Need

• 3 polyacrylamide gels (12%)
• MV10 vertical gel electrophoresis apparatus
• power supply
• microcentrifuge
• hot plate or burner
• vortex
• white light visualization system
• automatic micropipet with fine tips
• test tube holders
• lab glassware
• methanol
• glacial acetic acid
• distilled water.

 

Order Info

For six groups sharing three gels.

Recommended Storage

Some components require freezer storage

Specifications

• Product Type: Protein Kit
• Includes: Instructions, fish lyphoprotein™ samples, protein molecular weight standards, practice gel loading solution, buffer, protein instastain™
• Content And Storage: Refrigerated. Some components require freezer storage.

• For Use With (Application): Identify the specific species
• Time Range: 60 minutes; Staining time: 20 minutes
• Class Size: 6 Lab Groups