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Edvotek™ Cleavage of Lambda DNA with EcoRI
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for EcoRI (five distinct sites; two very close in size).
Supplier: Edvotek™ 212
Includes:
- Instructions
- Lambda phage DNA
- Concentrated Restriction Enzyme Reaction Buffer
- EDVOTEK™ Enzyme Grade Water
- Eco RI Dryzymes™ endonuclease
- DNA Standard Marker, Reconstitution Buffers
- UltraSpec-Agarose™
- 10X Gel Loading Solution
- 50X Concentrated Electrophoresis Buffer
- Practice Gel Loading Solution
- FlashBlue™ DNA Stain
- InstaStain™ Blue Cards
- Disposable Pipets
- Microtest Tubes
- Semi-log Graph Paper Template
Requires :
- Electrophoresis apparatus
- Power supply
- Automatic pipet with tips
- Water bath
- Balance
- Microwave or hotplate
- Visualization (white light)
- Lab glassware
- Pipet pumps or bulbs
- Metric rulers
- Floating racks
- Distilled or deionized water
- Ice
Description
- Students digest lambda DNA with EcoRI endonuclease
- Digestion products are analyzed by agarose gel electrophoresis
- Complete in 90 minutes
Order Info
Contains materials for 10 restriction digestions and five gels.
Recommended Storage
Some components require freezer storage
Specifications
| Cleavage of Lambda DNA with Eco Ri Endonuclease Kit | |
| Instructions, lambda dna, dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard dna fragments, various solutions and buffers, agarose powder, flashblue™ stain | |
| 90 min. |
| Digestion products are analyzed by agarose gel electrophoresis | |
| 10 Restriction Digestions and 5 Gels | |
| Refrigerated; Store at: -20°C; -4°F |
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