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Edvotek™ AP LAB 9: Biotechnology — Restriction Enzyme Analysis of DNA

Name: Edvotek&trade; AP LAB 9: Biotechnology &mdash; Restriction Enzyme Analysis of DNA
Brand: Edvotek&trade;
SKU: S06887
Price: 130.00 USD

For analysis of Eco RI cleavage patterns of lambda DNA

Supplier: Edvotek™ AP09

Catalog No. S06887

$130.00 / Each of 1

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Includes: Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.

Requires : Horizontal gel electrophoresis apparatus; DC power supply; automatic micropipets with tips; balance; microwave; hotplate or burner; pipet pump; 250mL flasks or beakers; hot gloves; safety goggles; disposable laboratory gloves; small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light) and distilled or deionized water

Description

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences
Bateriophage lambda DNA has a linear structure and six Eco RI recognition sites
Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield six bands (five distinct bands, two are very close in size) corresponding to the DNA fragments

Order Info

For six gels
Takes one hour to complete

Specifications

Product Type	Biotechnology Kit
Includes	Instructions, dna samples, ultraspec agarose™ powder, concentrated electrophoresis buffer, flashblue™ dna stain, instastain™ blue cards, practice gel loading solution, 1ml pipet, and microtipped transfer pipets
Time Range	60 min.

For Use With (Application)	Restriction enzyme analysis of dna
Quantity	8 Gels
Content And Storage	Refrigerated

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