Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Edvotek™ AP LAB 9: Biotechnology — Restriction Enzyme Analysis of DNA
For analysis of Eco RI cleavage patterns of lambda DNA
Supplier: Edvotek™ AP09
Includes: Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.
Requires : Horizontal gel electrophoresis apparatus; DC power supply; automatic micropipets with tips; balance; microwave; hotplate or burner; pipet pump; 250mL flasks or beakers; hot gloves; safety goggles; disposable laboratory gloves; small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light) and distilled or deionized water
Description
- This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences
- Bateriophage lambda DNA has a linear structure and six Eco RI recognition sites
- Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield six bands (five distinct bands, two are very close in size) corresponding to the DNA fragments
Order Info
- For six gels
- Takes one hour to complete
Specifications
| Restriction enzyme analysis of dna | |
| Biotechnology Kit | |
| 60 min. |
| Instructions, dna samples, ultraspec agarose™ powder, concentrated electrophoresis buffer, flashblue™ dna stain, instastain™ blue cards, practice gel loading solution, 1ml pipet, and microtipped transfer pipets | |
| 8 Gels | |
| Refrigerated |
Provide Content Correction
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Product Title