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Edvotek™ AP LAB 13: Enzyme Activity
Learn about enzyme catalysis and the nature of enzyme action.
Supplier: Edvotek™ AP13
Includes: Hydrogen peroxide solution, guaiacol solution, phosphate buffer pH 3, phosphate buffer pH 7, phosphate buffer pH 10 and phosphate buffer pH 14
Requires : Turnip root, distilled or dionized water, pipet pumps or bulbs, Erlenmeyer flask (500mL), spectrophotometer, water baths, filter paper and funnel, test tube racks, test tubes (13 × 150mm), thermometer, cheesecloth, Parafilm™ film, hot plate, timer or clock with second hand, lab permanent markers, ice, razor, goggles and blender
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This safe, easy experiment allows your students to learn about enzyme catalysis, the nature of enzyme action and protein structure-function relationships.
- Students perform an enzyme assay and determine the rate of the enzymatic reaction Experiment uses a safer system that eliminates the need for sulfuric acid and potassium permanganate
- Class size: 10 lab groups
- Requires 30 to 45 minutes
|To perform an enzyme assay and determine the rate of the enzymatic reaction|
|Enzyme Activity Kit|
|Instructions, hydrogen peroxide solution, guaiacol solution, phosphate buffer ph 3, phosphate buffer ph 7, phosphate buffer ph 10, and phosphate buffer ph 14|
|30 to 45 min.|
|10 Lab Groups|
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