Thermo Scientific™ DyLight™ Antibody Labeling Kits

Covalently label antibodies or proteins with DyLight dyes for use in immunofluorescence, flow cytometry, western blot analysis, and high content analysis.

Catalog Number	Mfr. No.	Excitation/Emission	Label or Dye
PI53020	Thermo Scientific™ 53020	400/420	DyLight 405
PI53024	Thermo Scientific™ 53024	490/525	DyLight 488
PI53044	Thermo Scientific™ 53044	590/617	DyLight 594
PI53046	Thermo Scientific™ 53046	638/658	DyLight 633
PI53056	Thermo Scientific™ 53056	679/702	DyLight 680
PI53062	Thermo Scientific™ 53062	784/814	DyLight 800
PI62275	Thermo Scientific™ 62275	346/442	DyLight 350
PI84530	Thermo Scientific™ 84530	562/576	DyLight 550
PI84535	Thermo Scientific™ 84535	652/672	DyLight 650
PI84538	Thermo Scientific™ 84538	749/775	DyLight 755

Description

Invitrogen Dylight Antibody Labeling kits provide everything needed to label 1 mg of antibody or protein with amine-reactive Dylight dye and subsequent free dye removal. Choose from a variety of Dylight fluorophores across the spectrum to covalently label your antibody or protein. Labeled antibodies are typically ready to use in 90 minutes with minimal hands-on time. The kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

Invitrogen DyLight Antibody Labeling kits enable the creation of fluorescent antibodies that are covalently linked to different DyLight fluorophores. The fluorescently labeled antibodies can be used in a wide variety of laboratory applications, including immunofluorescence microscopy, IHC, western blotting, ELISA, flow cytometry, or in vitro and in vivo fluorescent detection strategies.

DyLight Antibody Labeling kits contain an NHS ester-activated derivative of the high-performance DyLight dye that can be used to fluorescently label antibodies and other proteins for cellular imaging and other fluorescence detection methods. The DyLight dye is an amine-reactive dye and is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds.

DyLight Antibody Labeling kits contain all necessary components to perform three separate labeling reactions and free dye removal using 1 mg of IgG or similar quantities of other proteins.

DyLight dyes exhibit higher fluorescence intensity and photostability than CyDye and LI-COR™ dyes in many applications and remain highly fluorescent over a broad pH range (pH 4–9). Additionally, the water solubility of the DyLight fluorescent dyes allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. Finally, the high solubility of these fluorophores permits protein solutions to be added directly to specific amounts of the labeling reagent.

Other advantages of the DyLight Antibody Labeling kits:
· High performance—high fluorescence intensity across a broad pH range
· Multiple colors—10 different dyes across the visible spectrum and extending into the infrared
· Specificity—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH₂)
· Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

We also offer DyLight Microscale Antibody Labeling kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods. The DyLight Microscale Antibody Labeling kits contain all necessary components to perform five separate labeling reactions using 100 μg of IgG or similar quantities of other proteins. Both the standard and microscale kit sizes include the amine-reactive DyLight fluorophore in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Specifications

• Product Type: Labeling Kit
• Labeling Scale: 0.5 to 1 mg
• Chemical Reactivity: Amine
• Label Type: DyLight™

• Content And Storage: Store the DyLight NHS Ester at -20°C. Store all other kit components at 4°C.
• Product Line: DyLight™
• Labeling Target: Antibodies, Proteins