MilliporeSigma™ DNA-RNA Hybrid, Mouse, Unlabeled, Clone: S9.6,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABE1095

Catalog No. MABE1095MI

Description

DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.

Specifications

• Antigen: DNA-RNA Hybrid
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Immunogen: DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
• Quantity: 100 μg
• Research Discipline: Epigenetics & Nuclear Function
• Test Specificity: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: ChIP Assay, Dot Blot, Immunocytochemistry, Immunoprecipitation
• Clone: S9.6
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.